adipose tissue progenitor isolation kit (Miltenyi Biotec)
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adipose tissue progenitor isolation kit
Adipose Tissue Progenitor Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+tissue/Adipose+Tissue+Progenitor+Isolation+Kit%2C+mouse/pm42494310-195-9-14
Average 93 stars, based on 34 article reviews
Adipose Tissue Progenitor Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+tissue/Adipose+Tissue+Progenitor+Isolation+Kit%2C+mouse/pm42494310-195-9-14
Average 93 stars, based on 34 article reviews
adipose tissue progenitor isolation kit - by Bioz Stars,
2026-10
93/100 stars
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Isolation:Article Title: Mitochondria transfer-based therapies reduce the morbidity and mortality of Leigh syndrome. Article Snippet: Mitochondria transfer is a recently described phenomenon in which donor cells deliver mitochondria to acceptor cells.. One possible consequence of mitochondria transfer is energetic support of neighbouring cells; for example, exogenous healthy mitochondria can rescue cell-intrinsic defects in mitochondrial metabolism in cultured ρ cells or Ndufs4 peritoneal macrophages.. Exposing haematopoietic stem cells to purified mitochondria before autologous haematopoietic stem cell transplantation allowed for treatment of anaemia in patients with large-scale mitochondrial DNA mutations, and mitochondria transplantation was shown to minimize ischaemic damage to the heart, brain and limbs. Article Title: Keratins Modulate Cellular Reduction‒Oxidation Homeostasis and Mitochondrial Dynamics. Article Snippet: After washing, coverslips were dried and mounted in ProLong Gold Antifade mounting medium (Thermo Fisher Scientific). .. Mitochondria were isolated using Mitochondria Isolation Kit for Article Title: Mitochondrial translation termination, recycling, reinitiation, and rescue for in-frame and out-of-frame contexts Article Snippet: .. Then, mitochondrial purification was performed by immunoprecipitation with anti-TOM22 antibody-conjugated beads from a Mitochondria Isolation Kit, Human (Miltenyi Biotec) or a Mitochondria Isolation Kit, Article Title: Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue Article Snippet: Pre-Separation Filters (30 μm) , Miltenyi Biotec , Cat# 130–041-407. .. Mitochondria Isolation Kit, Article Title: Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in Obesity. Article Snippet: Expression of Ext1, Ext2, and Hs6st1 was measured using TaqMan assays on a 7500 fast real-time PCR machine (Applied Biosystems). .. Mitochondria Isolation Mitochondria were isolated using the Mitochondria Isolation Kit, Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method Article Snippet: .. Resource availability , Mitochondria Extraction Kit - Tissue | Miltenyi Biotec Mitochondria Isolation Kit, Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method Article Snippet: .. Mitochondria Extraction Kit – Tissue (Miltenyi Biotec, Cat#130-097-340) Mitochondria Isolation Kit, Article Title: Astrocytic PI3Kα controls synaptic plasticity and cognitive function via serine metabolism Article Snippet: After centrifugation at 300× g for 5 min at 4°C, pellets were resuspended in Protease Inhibition Buffer supplemented with EDTA-free protease inhibitor cocktail (Roche, #05892791001) and dissociated with a GentleMACS tissue dissociator (Miltenyi Biotec, #130-093-235). .. Homogenates were centrifuged at 500× g for 5 min at 4°C and the supernatants containing mitochondria were purified as directed in the Mitochondria Isolation Kit, Purification:Article Title: Mitochondrial translation termination, recycling, reinitiation, and rescue for in-frame and out-of-frame contexts Article Snippet: .. Then, mitochondrial purification was performed by immunoprecipitation with anti-TOM22 antibody-conjugated beads from a Mitochondria Isolation Kit, Human (Miltenyi Biotec) or a Mitochondria Isolation Kit, Article Title: Astrocytic PI3Kα controls synaptic plasticity and cognitive function via serine metabolism Article Snippet: After centrifugation at 300× g for 5 min at 4°C, pellets were resuspended in Protease Inhibition Buffer supplemented with EDTA-free protease inhibitor cocktail (Roche, #05892791001) and dissociated with a GentleMACS tissue dissociator (Miltenyi Biotec, #130-093-235). .. Homogenates were centrifuged at 500× g for 5 min at 4°C and the supernatants containing mitochondria were purified as directed in the Mitochondria Isolation Kit, Immunoprecipitation:Article Title: Mitochondrial translation termination, recycling, reinitiation, and rescue for in-frame and out-of-frame contexts Article Snippet: .. Then, mitochondrial purification was performed by immunoprecipitation with anti-TOM22 antibody-conjugated beads from a Mitochondria Isolation Kit, Human (Miltenyi Biotec) or a Mitochondria Isolation Kit, Sterility:Article Title: Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in Obesity. Article Snippet: Expression of Ext1, Ext2, and Hs6st1 was measured using TaqMan assays on a 7500 fast real-time PCR machine (Applied Biosystems). .. Mitochondria Isolation Mitochondria were isolated using the Mitochondria Isolation Kit, Extraction:Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method Article Snippet: .. Resource availability , Mitochondria Extraction Kit - Tissue | Miltenyi Biotec Mitochondria Isolation Kit, Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method Article Snippet: .. Mitochondria Extraction Kit – Tissue (Miltenyi Biotec, Cat#130-097-340) Mitochondria Isolation Kit, Magnetic Cell Separation:Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method Article Snippet: .. Resource availability , Mitochondria Extraction Kit - Tissue | Miltenyi Biotec Mitochondria Isolation Kit, Protease Inhibitor:Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method Article Snippet: .. Mitochondria Extraction Kit – Tissue (Miltenyi Biotec, Cat#130-097-340) Mitochondria Isolation Kit, Bicinchoninic Acid Protein Assay:Article Title: Purification of murine cortex mitochondria using a modified magnetic bead isolation method Article Snippet: .. Mitochondria Extraction Kit – Tissue (Miltenyi Biotec, Cat#130-097-340) Mitochondria Isolation Kit, |
![(a) Strategy used to obtain Mpc2 knockout astrocytes in primary culture. Created with BioRender.com. (b) Representative Western blot showing MPC2 and MPC1 protein levels 4, 6 and 8 days after AdV-CMV-Cre transduction in immunomagnetically purified <t>mitochondria</t> from primary astrocytes; HSP60 was used as loading control. (c) RNA-Sequencing in WT and Mpc2-KO primary astrocytes. Selected genes are shown. n=3 biologically independent samples per genotype; Wald test with local fitting. (d) [U- 13 C]Glucose tracing in WT and Mpc2-KO primary astrocytes. Left : heatmap and right : molecular carbon labeling (MCL) quantification of the detected metabolites. Data are mean ± S.E.M. P values are indicated; n=3 biologically independent samples per genotype; Unpaired Student’s t -test, two-tailed. (e) Glycolytic flux in WT and Mpc2-KO primary astrocytes. Data are mean ± S.E.M. P value is indicated; n=4 biologically independent samples per genotype; Unpaired Welch’s t -test, two-tailed. Non-normalized values are 384.76 ± 118.84 nmol x h −1 x mg protein −1 for WT and 445.49 ± 140.60 nmol x h −1 x mg protein −1 for Mpc2-KO astrocytes. (f) Pyruvate decarboxylation flux at the PDH in WT and Mpc2-KO primary astrocytes. Data are mean ± S.E.M. P value is indicated; n=3 biologically independent samples per genotype; Unpaired Welch’s t -test, two-tailed. Non-normalized values are 146.09 ± 57.26 nmol x h −1 x mg protein −1 for WT and 93.56 ± 37.46 nmol x h −1 x mg protein −1 for Mpc2-KO astrocytes. (g) Pyruvate decarboxylation flux at the TCA cycle in WT and Mpc2-KO primary astrocytes. Data are mean ± S.E.M. P value is indicated; n=5 biologically independent samples per genotype; Unpaired Welch’s t -test, two-tailed. Non-normalized values are 26.52 ± 5.65 nmol x h −1 x mg protein −1 for WT and 16.05 ± 3.05 nmol x h −1 x mg protein − 1 for Mpc2-KO astrocytes. (h) Alanine decarboxylation flux at the PDH in WT and Mpc2-KO primary astrocytes. Data are mean ± S.E.M. P value is indicated; n=3 biologically independent samples per genotype; Unpaired Welch’s t -test, two-tailed. Non-normalized values are 11.51 ± 2.18 nmol x h −1 x mg protein −1 for WT and 18.33 ± 3.12 nmol x h −1 x mg protein −1 for Mpc2-KO astrocytes. (i) Acetate decarboxylation flux at the TCA cycle in WT and Mpc2-KO primary astrocytes. Data are mean ± S.E.M. P value is indicated; n=3 biologically independent samples per genotype; Unpaired Welch’s t -test, two-tailed. Non-normalized values are 5.79 ± 1.90 nmol x h −1 x mg protein −1 for WT and 2.37 ± 0.51 nmol x h −1 x mg protein −1 for Mpc2-KO astrocytes. (j) Oxygen consumption rate (OCR) analysis in WT and Mpc2-KO primary astrocytes. Left : OCR traces; right : quantifications of basal respiration, maximal respiration and spare respiratory capacity. Data are mean ± S.E.M. P values are indicated; n=3 biologically independent samples per genotype; Unpaired Student’s t -test, two-tailed. (k) Analysis of the mitochondrial respiratory chain complex III superassembly. Left : Blue-native gel electrophoresis images showing complex III (CIII) containing supercomplexes (SC-CIII) and free CIII in WT and Mpc2-KO astrocytes. Right : Quantification of SC versus free CIII. Data are mean ± S.E.M. P value is indicated; n=4 biologically independent samples per genotype; Unpaired Student’s t -test, two-tailed.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_58/10__64898_slash_2026__07__04__736458/10__64898_slash_2026__07__04__736458___F3.large.jpg)
